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Figure 1

From: Microneedle-mediated immunization of an adenovirus-based malaria vaccine enhances antigen-specific antibody immunity and reduces anti-vector responses compared to the intradermal route

Figure 1

Influence of the microneedle patch design on the induction of antibody responses by a recombinant adenovirus vaccine.

C57BL/6 mice were immunized with 1 × 1010 vp HAdV5-PyMSP142 by the intradermal (ID) route or using a silicon microneedle patch of differing design, as outlined in Table I. All mice were boosted at 8 weeks with MVA-PyMSP142 by the ID route. Total IgG titers in blood were measured to P. yoelii MSP119 at 8 weeks after the primary immunization and 2 weeks after the boost. (A) Individual responses at 8 weeks post-prime. (B) Antigen-specific total IgG responses at 8 weeks post-prime (light grey bars) and 2 weeks post-boost (dark grey bars). * p < 0.05, ** p < 0.01, ***p < 0.001 compared to post-prime antibody responses, by 2way ANOVA with Bonferroni post-test.; ★ p < 0.05, ★★★ p < 0.001 compared to ID immunization by one way ANOVA using Dunn's multiple comparison test. IgG1(light grey bars) and IgG2a (dark grey bars) antibody responses to the antigen were measured in the serum (C) 8 weeks post-prime and (D) 2 weeks after MVA-PyMSP142 boosting. The mean and standard error of the mean (SEM) (n = 5 to 17 mice) are shown in all panels.

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