Figure 6 | Scientific Reports

Figure 6

From: Microneedle-mediated immunization of an adenovirus-based malaria vaccine enhances antigen-specific antibody immunity and reduces anti-vector responses compared to the intradermal route

Figure 6

The route of immunization impacts on vaccine efficacy.

C57BL/6 mice were immunized twice (day 0 and 56) with 1 × 1010 vp HAdV5-PyMSP142 by the ID route or using a silicon microneedle patch type D (MN) (Ad/Ad) using the same (ID/ID, MN/MN) route in both immunizations or using a MN patch in the prime and boosting by the ID route (MN/ID). Alternatively, mice primed with HAdV5-PyMSP142 were boosted with 1 × 106 pfu MVA-PyMSP142 by the ID route (Ad/MVA). (A) Two weeks post-boost all immunized mice or naïve controls were challenged i.v. with 1 × 104 pRBCs. Parasitemia was measured as described in Materials and Methods from day 2 after challenge. The number of surviving mice, of a total of 6 mice per group, is indicated. Crosses indicate when mice were sacrificed.

Back to article page