Figure 1
From: Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells
(A) Cyclic voltammograms (from +0.5 V to −1.0 V) were obtained in aqueous 50 mM phosphate buffer at pH 5.5 and 3.5 for the electrochemical reduction of human haemoglobin (0.05 mg/mL, 0.78 μM) at GC electrodes. Reduction peak potential of haemoglobin decreases with an increase in pH. Scan rate is 0.1 Vs−1. Inset: Reduction peak height of cyclic voltammograms at different pH values. Error bars represent RSD (n = 3; 95% confidence interval). (B) Cyclic voltammograms (from +0.5 V to −1.0 V) were obtained in aqueous 50 mM phosphate buffer for the electrochemical reduction of blood at pH 3.5 and pH 7.4. Blood concentration was chosen to be 1 × 103 cells/μL. Reduction peak current magnitudes decreases with an increase in pH. Scan rate is 0.1 Vs−1. Inset: Reduction peak height of cyclic voltammograms at different pH values. Error bars represent RSD (n = 3; 95% confidence level).
