Figure 6

Effects of RR-ARFs on IKK and MAPK signaling in LPS/IFN-γ-stimulated RAW264.7 cells.

Cells were stimulated with LPS/IFN-γ in the absence or presence of RR-ARFs (200 μg/ml) for the indicated time. The protein levels of phospho-IKKα/β (85–87 kDa), IKKα (85 kDa), IKKβ (87 kDa), phospho-IκBα (40 kDa), IκBα (39 kDa), phospho-p65 (65 kDa), p65 (65 kDa), phospho-p38 (43 kDa) and phospho-JNK (46 and 54 kDa) were detected by Western blot analyses using their respective antibodies (a and b). β-actin was used as an internal loading control. The full-length blots with those antibodies were presented in supplementary Figure S2. All gels have been run simultaneously under the same experimental conditions. Cell culture experiments were performed at least three times. Western blot data was quantitated to better view the difference between different treatment groups. Data represent means ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001 (c).