Table 3 Cardiac expression of glucose and FA metabolism related genes measured by RT-PCR

From: Lack of cardiac and high-fat diet induced metabolic phenotypes in two independent strains of Vegf-b knockout mice

pathway/metabolism

gene

Aase WT

Aase Vegf-b-/-

Bellomo WT

Bellomo Vegf-b-/-

glucose

Pdha1

2.729 ± 0.32

3.051 ± 0.16

3.231 ± 0.26

3.093 ± 0.30

 

Pdk4

3.037 ± 0.39

3.737 ± 0.48

2.986 ± 0.31

2.422 ± 0.15

 

Glut4(Slc2a4)

2.603 ± 0.28

3.064 ± 0.18

2.969 ± 0,32

3.005 ± 0.23

FA oxidation

Acadm

1.144 ± 0.07

1.203 ± 0.16

0.977 ± 0.11

1.104 ± 0.08

 

Acadl

1.244 ± 0.12

1.293 ± 0.17

1.022 ± 0.12

1.181 ± 0.09

 

Cpt1β

1.106 ± 0.08

1.321 ± 0.24

0.939 ± 0.14

1.023 ± 0.10

FA transport

Fatp3(Slc27a3)

1.070 ± 0.06

1.304 ± 0.16

0.912 ± 0.12

0.926 ± 0.12

 

Fatp4(Slc27a4)

1.303 ± 0.11

1.445 ± 0.22

0.912 ± 0.10

1.015 ± 0.08

FA synthesis

Fasn

1.110 ± 0.08

1.135 ± 0.15

0.891 ± 0.11

1.005 ± 0.08

co-factors

Pgc-1α

2.521 ± 0.45

3.026 ± 0.16

3.350 ± 0.36

2.954 ± 0.39

 

Ncor1

2.497 ± 0.40

3.145 ± 0.26

3.496 ± 0.26

3.527 ± 0.58

 

Pparα

2.545 ± 0.43

2.859 ± 0.12

3.192 ± 0.36

3.213 ± 0.26

  1. Pdha1,pyruvate dehydrogenase alpha 1; Pdk4, pyruvate dehydrogenase kinase, isozyme 4; Slc2a4/Glut4, glucose transporter 4; Acadm, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; Acadl, acyl-Coenzyme A dehydrogenase, long chain; Cpt1β, carnitine palmitoyltransferase I beta; Slc27a3/Fatp3, fatty acid transport protein 3; Slc27a4/Fatp4, fatty acid transport protein 4; Fasn, fatty acid synthase; Pgc-1α, peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; Ncor1, nuclear receptor co-repressor 1; Pparα, peroxisome proliferator-activator receptor alpha. Messenger RNA expression of indicated genes was normalized to B2M or PPIA. Results are expressed as mean ± S.E.M. n = 5–7.
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