Figure 2
From: Unequivocal identification of intracellular aluminium adjuvant in a monocytic THP-1 cell line
Representative autofluorescence controls of agar-paraffin embedded THP-1 cells (2 μm sections) co-cultured with 200 μg/mL AlO(OH)Sigma adjuvant (24 h).
Cell sections were incubated for 24 h in 50 mM PIPES, pH 7.4. Slides were mounted with ProLong® Gold Antifade Reagent with DAPI. Inserts show close-ups of individual cells. (a). autofluorescence (green), (b). DAPI-staining (blue), (c). autofluorescence & DAPI overlay, (d). autofluorescence, DAPI & light overlay. Magnification × 1000, scale bars: 20 μm.
