Figure 2

Postoperative indocyanine green fluorescence (ICG) angiography of the anorectal graft (normal dog: (a)–(d), dog 1: (e)–(h) and dog 4: (i)–(l)). (a), (e) and (i). Perineal view of the anal segment immediately before angiography. (b), (f) and (j). Beginning of enhancement of the anal segment. (c), (g) and (k). Plateau of enhancement. (d), (h) and (l). Time course of brightness determined using region of interest software (red line: positive control, blue line: negative control, yellow line: anal canal and green line: perianal skin). X-axis is a time scale and Y-axis represents brightness of the tissue.