Figure 2
From: Real Time Ligand-Induced Motion Mappings of AChBP and nAChR Using X-ray Single Molecule Tracking
DXT analysis procedures from diffraction spot trajectory to a 2D-internal motion map.
(a): Schematics of the diffraction spot trajectories in time-resolved diffraction images. Three examples of diffraction spot trajectories from gold nanocrystals exhibiting simple twisting motion (blue), simple tilting motion (green) and both tilting and twisting motions (red) are shown. (b–c): The displacement of angular position as a function of time in the tilting (b) and twisting (c) directions. (d): 2D scatterplot of the protein's internal motion generated from angular displacement in both the tilting and twisting directions in fixed time interval, e.g., 100 μs. (e): 2D-internal motion map of the protein. Logarithm of absolute displacement within a certain time interval was used to draw the 2D-histogram, which was normalised to obtain the 2D-internal motion map (probability density map).
