Figure 6
Histological and immunohistochemical analysis of kidneys.
Representative histopathology of kidney sections harvested from mice at 14 weeks of age. SCG/Kj mice were fed a control diet (A, C, E) or a 5% EPA-containing diet (B, D, F) beginning at age 7 weeks. The diets continued for 7 weeks and the mice were euthanized at 14 weeks of age. A and B, Masson-Trichrome staining (original magnification 100×). C and D, Masson-Trichrome staining (original magnification 200×). E and F, immunostaining of endothelial cells with anti-CD34 antibody (original magnification 200×). Arrows in Figure 6E and F indicate the staining of CD34 positive endothelial cells in glomeruli and interstitium in the kidney. SCG/Kj mice fed a 5% eicosapentaenoic acid-containing diet had less crescentic glomerulonephritis, less neutrophil accumulation into the glomeruli and restored endothelial cell damage in the peritubular capillaries.
