Figure 7

Fluorescence-activated cell sorting analysis of kidney and spleen T cell populations.

Lymphocyte profiling in the spleens and lymph nodes of SCG/Kj mice fed a control diet (open bar) or a 5% EPA-containing diet (closed bar). Single cell suspensions from the spleens and the lymph nodes of the kidneys (KLN) or the lungs (LLN) were stained with anti-CD3, anti-CD4, anti-CD8, or anti-B220 antibodies and anti-Foxp3 antibody. Lymphoproliferation in SCG/Kj mice is characterized by the accumulation of Thy-1⁺, Ly-1⁺, CD4/CD8 double negative and B220⁺ T cells. Representative dot plots are shown on the left. The percentages of CD3⁺B220⁺, CD3⁺Foxp3⁺ and CD3⁺CD4⁻CD8⁻ cells in CD3⁺ cells, were determined. The expression of these cells was analyzed by flow cytometry. Results are expressed as mean ± SEM percentages of CD3⁺B220⁺, CD3⁺CD4⁻CD8⁻ and CD3⁺Foxp3⁺ cells in the spleen and KLN and LLN of three mice per group fed a control diet (open bar) or a 5% EPA-containing diet (closed bar). (*p < 0.05 versus control by Student's t-test).