Figure 1 | Scientific Reports

Figure 1

From: An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System

Figure 1

Schematic overview of PAGE-based genotyping protocol for identification of CRISPR/Cas9-mediated indel mutations.

(A) Illustration of heteroduplex DNA formation during denaturation and annealing. Dark green bars represent four DNA strands (a–d) in cells harboring monoallelic mutations (orange box). After denaturation and annealing, two types of homoduplex DNA and two types of heteroduplex DNA were formed. (B) Identification of heteroduplex DNA fragments by 15% PAGE assay. Since heteroduplex DNA migrates slower due to formation of an open angle between matched and unmatched genomic regions, homoduplex DNA and heteroduplex DNA are easily identified based on their mobility rate.

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