Table 1 Summary of mouse embryo microinjection of Cas9 mRNA with different sgRNAs and number of mutant pups identified using the PAGE-based genotyping assay
Gene name | Cas9 (ng/μl) | sgRNA (ng/μl) | Injected zygotes | Newborns | Mutant pups identified with PAGE assay |
|---|---|---|---|---|---|
Agbl1 | 150 | 100 | 116 | 13 | 5 |
Agbl2 | 150 | 100 | 116 | 13 | 4 |
Agbl3 | 150 | 100 | 121 | 14 | 9 |
Agbl5 | 150 | 100 | 64 | 5 | 3 |
Nmi | 150 | 100 | 180 | 32 | 26 |
Them2 | 150 | 100 | 235 | 19 | 16 |
