Figure 4 | Scientific Reports

Figure 4

From: Reduced miR-3127-5p expression promotes NSCLC proliferation/invasion and contributes to dasatinib sensitivity via the c-Abl/Ras/ERK pathway

Figure 4

Oncogene c-Abl is a direct target of miR-3127-5p.

(A): Two sites in the ABL1 3′-UTR are complementary to miR-3127-5p as analyzed by Targetscan. (B): 293T cells were co-transfected with pmiR-RB-Report-ABL1_3′UTR or pmiR-ABL1_3′UTR-Mut plasmids and pL-GFP, pL-miR-3127-5p, or pL-miR-3127-5p-off plasmids. A Renilla-TK luciferase reporter (5 ng) was added as an internal control. The luciferase activity of the cells was determined after 48 h. (C): A549 or H292 cells transduced with Lv-3127, Lv-3127-off, or Lv-GFP were subjected to immunoblotting for c-Abl (The full-length blots were presented in the supplementary Figure S4). (D): H292-3127 was transducted with pGV142-CMV-ABL1 and control vectors, the cell cycle analysis and immunoblotting of c-abl were performed at 36hrs after eletroporation. (E): Wound scratch assay (left panel) and transwell invasion assay (right panel) of H292-3127 transduced with pGV142-CMV-ABL1 (Magnification, ×200). Triplicate experiments were done. (F): Left panels: Representative photographs of c-Abl IHC staining grades in NSCLC tumor samples (200×). Right panels: miR-3127-5p expression was low in c-Abl IHC positive staining samples (mean ± SEM, ANOVA).

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