Figure 6
between expression of miR-3127-5p and response to dasatinib in NSCLC cell lines.
(A): Dasatinib sensitivity of A549 (left) and H292 (right) cells transduced with Lv-3127, Lv-3127-off, or Lv-GFP as determined in a CCK8 cell viability assay. (p < 0.01, repeated measure ANOVA). (B): miR-3127-5p expression in the 14 NSCLC cell lines as determined using an miRNA poly (A) tailing-based Sybr Green Q-PCR assay. (C): Immunoblotting of c-Abl protein for NSCLC cell lines. The number under each band refers to the integrated band density (The full-length blots were presented in the supplementary Figure S6). (D): Negative relationship between c-Abl protein and miR-3127-5p expression in the NSCLC cell lines without K-Ras condon 12 mutation (Pearson's correlation, p < 0.01, r2 = 0.443). (E): IC50 values for dasatinib in NSCLC cell lines determined by CCK8 cell viability assay. (F): miR-3127-5p expression level was linearly associated with dasatinib IC50 for the 11 NSCLC cell lines after exclusion of the three cell lines with a K-Ras codon 12 mutation (K-Ras mutation status: H23(G12C), H358(G12C), A549(G12S), H460(Q61H), SPC-A1(Q61H). Pearson's correlation, p < 0.01, r2 = 0.895).
