Table 3 Differentially expressed proteins in normal and abortive chrysanthemum embryos
Protein (taxonomy) | AE18:NE12 | AE18:NE18 | NE18:NE12 | Function/category | Peptide number |
|---|---|---|---|---|---|
Cysteine synthase-like [Glycine max] | 1.69 ± 0.11 | 1.41 ± 0.03 | 1.20 ± 0.05 | Cell senescence and death | 2 |
Pathogenesis-related protein [Zinnia violacea] | 2.56 ± 0.10 | 3.09 ± 0.15 | 0.83 ± 0.02 | Cell senescence and death | 2 |
Selenium-binding protein 2 [Arabidopsis thaliana] | 1.51 ± 0.05 | 1.37 ± 0.04 | 1.11 ± 0.01 | Embryo sac development arrest | 5 |
Superoxide dismutase, chloroplastic [Cu-Zn] [Solidago canadensis var. scabra] | 2.51 ± 0.10 | 2.76 ± 0.10 | 0.91 ± 0.02 | Reactive oxygen species scavenger | 5 |
Ascorbate peroxidase 2-like protein [Tragopogon porrifolius] | 2.09 ± 0.04 | 2.38 ± 0.06 | 0.88 ± 0.02 | Reactive oxygen species scavenger | 3 |
Putative polyubiquitin 2 [Oryza sativa Japonica Group] | 1.34 ± 0.05 | 1.54 ± 0.04 | 0.87 ± 0.02 | Protein degradation | 6 |
Ubiquitin-fold modifier 1 [Arabidopsis thaliana] | 1.75 ± 0.08 | 1.87 ± 0.05 | 0.93 ± 0.02 | Protein degradation | 3 |
Proteasome subunit alpha type, putative [Ricinus communis] | 2.20 ± 0.10 | 2.05 ± 0.04 | 1.07 ± 0.03 | Protein degradation | 3 |
Calmodulin-7 [Arabidopsis thaliana] | 2.55 ± 0.13 | 2.40 ± 0.09 | 1.06 ± 0.02 | Calcium ion binding | 9 |
Polcalcin [Artemisia vulgaris] | 1.74 ± 0.09 | 1.93 ± 0.04 | 0.90 ± 0.03 | Calcium ion binding | 7 |
Cyclophilin, putative [Ricinus communis] | 1.75 ± 0.07 | 1.79 ± 0.06 | 0.98 ± 0.01 | Protein folding | 3 |
putative DNA repair protein RAD23-4 [Arabidopsis thaliana] | 1.69 ± 0.06 | 1.91 ± 0.06 | 0.89 ± 0.01 | DNA repair | 5 |
Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase [Arabidopsis thaliana] | 1.82 ± 0.13 | 1.56 ± 0.05 | 1.16 ± 0.05 | RNA metabolic process | 4 |
glycine-rich RNA binding protein [Oryza sativa Japonica Group] | 1.52 ± 0.07 | 1.27 ± 0.05 | 1.19 ± 0.01 | RNA transcription or processing during stress | 1 |
Omega-3 fatty acid desaturase, endoplasmic reticulum [Arabidopsis thaliana] | 1.85 ± 0.09 | 1.40 ± 0.03 | 1.32 ± 0.04 | Fatty acid biosynthesis | 2 |
Translationally-controlled tumor protein homolog [Arabidopsis thaliana] | 1.55 ± 0.06 | 1.43 ± 0.04 | 1.09 ± 0.02 | Microtubule binding | 3 |
Histone 1 [Malus domestica] | 1.40 ± 0.11 | 1.57 ± 0.04 | 0.89 ± 0.05 | Chromosome constituent | 1 |
Histone H2B.6 [Arabidopsis thaliana] | 1.33 ± 0.09 | 1.55 ± 0.06 | 0.86 ± 0.03 | Chromosome constituent | 6 |
Histone H4 [Glycine max] | 1.28 ± 0.06 | 1.60 ± 0.03 | 0.80 ± 0.02 | Chromosome component | 5 |
Prohibitin-2, mitochondrial [Arabidopsis thaliana] | 1.40 ± 0.09 | 1.51 ± 0.04 | 0.92 ± 0.04 | Chaperone for respiration chain proteins | 10 |
Actin-7 [Arabidopsis thaliana] | 0.78 ± 0.06 | 0.76 ± 0.03 | 1.01 ± 0.05 | Cytoskeleton constituent | 8 |
Tubulin alpha-6 chain [Arabidopsis thaliana] | 0.63 ± 0.02 | 0.78 ± 0.02 | 0.80 ± 0.01 | Cytoskeleton constituent | 5 |
Beta-tubulin [Zinnia violacea] | 0.67 ± 0.03 | 0.74 ± 0.03 | 0.90 ± 0.00 | Cytoskeleton constituent | 5 |
40S ribosomal protein S15a-1 [Arabidopsis thaliana] | 0.43 ± 0.01 | 0.58 ± 0.02 | 0.73 ± 0.00 | Ribosome constituent | 6 |
Ribosomal protein S3, component of cytosolic 80S ribosome and 40S small subunit [Ostreococcus lucimarinus (strain CCE9901)] | 0.64 ± 0.03 | 0.75 ± 0.03 | 0.86 ± 0.00 | Ribosome constituent | 5 |
putative ATP synthase beta chain [Oryza sativa (japonica cultivar-group)] | 0.66 ± 0.03 | 0.72 ± 0.02 | 0.91 ± 0.01 | Energy metabolism | 3 |
V-type proton ATPase catalytic subunit A [Daucus carota] | 0.82 ± 0.03 | 0.68 ± 0.0 1 | 1.20 ± 0.02 | Energy metabolism | 9 |
UDP-glucose 6-dehydrogenase [Oryza glaberrima] | 0.69 ± 0.03 | 0.72 ± 0.02 | 0.95 ± 0.02 | Energy metabolism | 4 |
2-oxoglutarate dehydrogenase, E1 subunit-like protein [Arabidopsis thaliana] | 0.80 ± 0.06 | 0.71 ± 0.02 | 1.11 ± 0.05 | Energy metabolism | 3 |
Aconitate hydratase [Arabidopsis thaliana] | 0.64 ± 0.03 | 0.74 ± 0.02 | 0.85 ± 0.03 | Energy metabolism | 7 |
Pyruvate kinase [Arabidopsis thaliana] | 0.75 ± 0.03 | 0.77 ± 0.03 | 0.97 ± 0.01 | Energy metabolism | 4 |
Fructose-bisphosphate aldolase [Medicago truncatula] | 0.77 ± 0.03 | 0.78 ± 0.02 | 0.98 ± 0.02 | Energy metabolism | 5 |
Glyceraldehyde 3-phosphate dehydrogenase (Fragment) [Salvia elegans] | 0.73 ± 0.03 | 0.60 ± 0.01 | 1.21 ± 0.03 | Energy metabolism | 8 |
Elongation factor EF-2 [Arabidopsis thaliana] | 0.71 ± 0.05 | 0.74 ± 0.02 | 0.96 ± 0.05 | Protein biosynthesis | 23 |
Elongation factor 1-alpha 1[Arabidopsis thaliana] | 0.78 ± 0.03 | 0.78 ± 0.01 | 1.00 ± 0.03 | Protein biosynthesis | 11 |
Eukaryotic initiation factor 4A [Oryza sativa Japonica Group] | 0.79 ± 0.02 | 0.70 ± 0.01 | 1.13 ± 0.01 | Protein biosynthesis | 7 |
Glutamyl-tRNA synthetase, cytoplasmic, putative [Ricinus communis] | 0.54 ± 0.02 | 0.47 ± 0.02 | 1.13 ± 0.00 | Protein biosynthesis | 2 |
Methionine synthase [Helianthus annuus x Helianthus debilis subsp. Debilis] | 0.65 ± 0.05 | 0.69 ± 0.01 | 0.94 ± 0.06 | Amino-acid biosynthesis | 6 |
14-3-3-like protein 2 [Gossypium hirsutum] | 0.48 ± 0.02 | 0.71 ± 0.01 | 0.67 ± 0.01 | ATP binding | 5 |
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase [Solenostemon scutellarioides] | 0.55 ± 0.02 | 0.62 ± 0.02 | 0.88 ± 0.01 | Methyltransferase | 12 |
Putative S-adenosylmethionine synthetase [Capsicum annuum] | 0.66 ± 0.02 | 0.74 ± 0.02 | 0.90 ± 0.01 | Methionine adenosyltransferase activity | 3 |
