Figure 11

The qPCR validation and expression analysis of 8 genes with up-methylated and down-regulated in several bovine tissues and organs.
The mRNA expression was normalized using ACTB and GAPDH and expressed relative to gene expression in the fetal bovine group (green bars) are given as a negative control. Green bars: fetal bovine; Blue bars: newborn bovine; Yellow bars: adult bovine. Error bars represent standard error of the mean (SE). Each column values represent the means ± SE of three replicates.