Figure 1
From: Introduction of impermeable actin-staining molecules to mammalian cells by optoporation
(a) Experimental setup for femtosecond laser-assisted optoporation. L: Ti: Sapphire laser; BE: beam expander; S: shutter; P: polarizer; Fl.: fluorescence excitation source; Ex: excitation filter; Em: emission filter; MO: microscope objective; CL: condenser lens; DM1 and DM2: dichroic mirrors; M: mirror; WL: halogen lamp. (b–c) Symmetric laser spot focused above and at the sample's surface plane. (d) Rat cortical neuron approximately 30 minutes following fs laser optoporation and injection with rhodamine phalloidin. (This figure was drawn by author (KD) in Microsoft PowerPoint software.).
