Figure 3 | Scientific Reports

Figure 3

From: Identification and characterization of OSTL (RNF217) encoding a RING-IBR-RING protein adjacent to a translocation breakpoint involving ETV6 in childhood ALL

Figure 3

Expression of RNF217 in human tissues and B cells.

(a) Multiple human tissue northern blot hybridized with a radioactively labeled RNF217 probe. (b) Cell lines derived from human B cell neoplasms were analyzed for their expression of RNF217 by RT-PCR. TBP expression was assessed as a control. The primers that were used for this experiment covered exons 9 and 11 of the RNF217 gene (see Supplementary table 1), to allow for the detection of all putative protein-coding splice variants shown in Supplementary table 2. Note that the RNF217 gel picture is a composite of two different gels. (c) Determination of RNF217 expression and splicing during B cell development. GAPDH expression was assayed as control.

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