Figure 3
Two-colour imaging under xenon lamp excitation.
(a) and (b) are non-time-gated and time-gated images of Giardia lamblia cysts labelled with a red europium probe and Cryptosporidium parvum oocysts labelled with a green terbium probe (exposure time: 5 seconds). (c) and (d) show the pixel intensity histograms for the signal area (target cells) and the background area in separate red and green channels for (a) and (b), respectively. (e) is a bar chart showing the average signal and background levels summarised from 10 pairs of non-time-gated and time-gated dual-colour images. Error bars represent ±1 s.e.m.
