Figure 3

(a) Gene Expression of Day 14 Differentiated MDSCs. Muscle-specific gene expression of MDSCs is dependent on culture conditions. Values are expressed as fold change in gene expression and normalized to 2D differentiation protocol (2D). Red line indicates twofold change. *P < 0.05. **P < 0.001. (b) NKX2-5 protein expression in 4F-AG-AMT and AMT was detected by immunohistochemistry.