Figure 4 | Scientific Reports

Figure 4

From: Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells

Figure 4

Histological Analysis of Day 14 Differentiated MDSCs.

(a) Low magnification image of AMT showing α-Actinin expression throughout the construct, indicating MDSCs acquire a muscle phenotype. (b) Expression of α-Actinin and TNNT2 in MDSCs differentiated under various protocols. Scale bars indicate 10 μm or 20 μm as stated in each image. Arrows point to multinucleated cells. Blue indicates nuclear DAPI staining. (c) Percentage of multinucleated cells (n > 4 nuclei). 4-factor treatment significantly reduced the percentage of multinucleated cells. ** P < 0.001.

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