Figure 6 | Scientific Reports

Figure 6

From: Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells

Figure 6

Intercellular Coupling of MDSCs.

(a) Intracellular calcium transients of spontaneously beating MDSC-CMs recorded using Rhod-2 AM dye from 4 different locations. Trace shows that the transients begin and end in a synchronous manner (vertical lines). (b) Co-staining of 4-factor treated day 14 4F-AG-AMT with GJA1 (red), α-Actinin (green) and DAPI (blue). Arrows show gap junction formation between cells. Scale indicates 10 μm. (c) Co-staining of day 14 2D Differentiated MSDCs with GJA1 (red), α-Actinin (green) and DAPI (blue). Scale bar indicates 10 μm.

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