Figure 6
Intracellular ROS in A549 cells following plasma treatment.
A549 cells were treated with DCF-DA and assayed using fluorescence microscopy. Intracellular ROS formation in response to helium plasma-exposed A549 cells for different oxygen flow rate: (A) Fluorescence images (upper row) of intracellular ROS concentration in A549 cells (non-treated, He plasma-treated, He/O2 plasma-treated) and bright-field images (lower row), (B) the quantification by measuring fluorescence pixel intensity with MetaMorph software (Each point represents the mean ± SD of three replicates. * p < 0.05 and ** p < 0.01). Scale bar = 100 μm.
