Figure 3
Effects of temperature and pH on activity of rhGPx3 mutant.
(a) Plot of GPx activity versus temperature. The activities were measured at the temperature range of 15–55°C at pH 7.4. The enzyme activity was assumed as 100% at 45°C. (b) Plot of GPx activity versus pH. The activities were measured in the pH range of 4.7–9.8 at 37°C. The enzyme activity was assumed as 100% at pH 8.9. The concentration of GSH and H2O2 used for assay was 1 mM and 0.5 mM, respectively. The scale of relative activity (%) indicates the percentage of the activity relative to the highest activity (100%) within each experiment.
