Figure 4

Changed transcription patterns of SSR-containing genes upon autopolyploidization.
No amplicons were produced from either the diploid or any of the four tetraploid C. nankingense cDNA templates using primer pairs #14 or #15, while primer pairs #2 and #31 in autotetraploid C. nankingense and primer pairs #31 and #15 in autotetraploid C. lavandulifolium produced an identical amplicon from all five templates. However, primer pair #26 detected transcription changes in two (T1 and T3) of the four tetraploid C. nankingense plants and primer pair #3 detected transcription changes in three of six autotetraploid C. lavandulifolium plants. Primer pair #34 detected transcription in all autotetraploid but not in the diploid. The cropped gels have been run under the same experimental conditions. Full-length gels are presented in Supplementary Fig. S3.