Table 2 Primer sequences employed to assay variation at 20 EST-SSR loci
Primers ID | SSR motifs | Primer sequence (5′-3′) | Polymorphic |
|---|---|---|---|
#1 | CAT | F: TTCCTCCTATAGCCAAGGCA | Â |
| Â | Â | R: GGCTGGATCAGTCGTTTCAT | Â |
#2 | ACC | F: AAACCACCAAACCCATCAAA |
|
| Â | Â | R: AACTTTGCCAGCATCGACTT | Â |
#3 | GTG | F: CGACAGTTGAGCAACAGGAA |
|
| Â | Â | R: CCAATCACCGACACATCATC | Â |
#4 | CAC | F: TATCCCACTATGCCCACCAC | Â |
| Â | Â | R: AAACTGTGGTGCATGGTCAA | Â |
#5 | CACCTG | F: GGCACAGCAACTTCAAGACA | Â |
| Â | Â | R: ATTTTCTGCGGCATTTTCTG | Â |
#6 | AAGAGG | F: GGAAGAGGAGGAAGAGGACG | Â |
| Â | Â | R: CGTTTAGGGCTGCTTCTTTG | Â |
#7 | GAATGT | F: ATGGCATTGAGGAGGATGAG | Â |
| Â | Â | R: AACATTACCCGCACCAACAC | Â |
#8 | GGGTCA | F: CCAACAACCACAACAACAGC | Â |
| Â | Â | R: ACAGAACCATTATGCGGAGC | Â |
#9 | TGA | F: CAAAAGTGCAAATGGAGGCT | Â |
| Â | Â | R: TCCCCAAATAACAAACCAACA | Â |
#10 | CCT | F: AACTTACCAACCCCACTCCC | Â |
| Â | Â | R: AACACTGGTCTCCAATTCCG | Â |
#11 | AAG | F: GTTCACCGTCAATTCACACG | Â |
| Â | Â | R: ATGTTTCGACAGTCGCAGTG | Â |
#12 | TCA | F: GAGCGCAGGAAGAAGAGAAA | Â |
| Â | Â | R: GCGCTTCTTCTCACGTCTCT | Â |
#13 | ATC | F: CCCCAATTCCTGTATCATCA | Â |
| Â | Â | R: ATCAGCTCGGGCTCAATCTA | Â |
#14 | GAA | F: CACCCTCTTCATGTCAAAACC |
|
| Â | Â | R: ACCATTTTCATCCACCCAAT | Â |
#15 | GTG | F: CCGGTGTTCGGTATAAATGG |
|
| Â | Â | R: ACAATTCGCTTCGGCTCTAA | Â |
#16 | CAA | F: ATGGGGAGATATGCCCTTTC | Â |
| Â | Â | R: TAAGTAAATCTGCCCTGGCG | Â |
#17 | GCT | F: GGAATATTCGACCGGGATTT | Â |
| Â | Â | R: ACTGAATTTGCACAGTCCCC | Â |
#18 | AAG | F: TTCCCCATGAAGAGGAACTG | Â |
| Â | Â | R: TCTGATGTAGGCATAGATAGCACA | Â |
#19 | GAG | F: TCGTTAAAATGGCGTCATCA | Â |
| Â | Â | R: TCATTCCTATCGCCAAAACC | Â |
#20 | ATG | F: GACGACGACGACAATGAAGA | Â |
| Â | Â | R: CTTGATGGGAAACCGTCTGT | Â |
#21 | CAA | F: TGTCGATCATCCCAAGTTTC | Â |
| Â | Â | R: TTTAAGCCCTGGTACATCGG | Â |
#22 | CTT | F: CACCGGAATTGTAACCAACC | Â |
| Â | Â | R: GCAAATCACTTGCTCCAACA | Â |
#23 | TCC | F: CCCTTTTGTCTCAAAGAACACA | Â |
| Â | Â | R: GCACGTTGTAAAGAATGCGA | Â |
#24 | ACC | F: TCTATCTCACCGGTCACACG | Â |
| Â | Â | R: GTGCGTCCAAACATTGAGTG | Â |
#25 | TCA | F: AACCATGAATCCAGACACCC | Â |
| Â | Â | R: ACCAAGCCAGTCGAGTTTTG | Â |
#26 | TTC | F: GGCGATGGATGATGATGATT |
|
| Â | Â | R: GAAAGAGGTGGATCGGATGA | Â |
#27 | AAG | F: CTAGTCGTGCTCCTCAGGCT | Â |
| Â | Â | R: AGTTCGGCCATGACATTTTC | Â |
#28 | GAATGT | F: CGTTATCCCAATCGTCGTCT | Â |
| Â | Â | R: AACATTACCCGCACCAACAC | Â |
#29 | GGGTCA | F: CCAACAACCACAACAACAGC | Â |
| Â | Â | R: ACAGAACCATTATGCGGAGC | Â |
#30 | TCA | F: TCAATGGACATAAAAGAAAGA | Â |
| Â | Â | R: GGTGGACAATCCGGTCATAC | Â |
#31 | TTC | F: GATTTCCCGATCGAATCAGA |
|
| Â | Â | R: TACAACGGACGGTGCATAGA | Â |
#32 | ATC | F: CCCCAATTCCTGTATCATCA | Â |
| Â | Â | R: ATCAGCTCGGGCTCAATCTA | Â |
#33 | CAC | F: ACCATGGACGCCATATCAAC | Â |
| Â | Â | R: TGGTGCATATTGCATGGTCT | Â |
#34 | A(G)AGATG | F: CCTAGTATCAAAGCTGCGAACA |
|
| Â | Â | R: CAATCGCGTTATCGTGTACC | Â |
#35 | CCA | F: CTTGACTTCAGCGACCATCA | Â |
| Â | Â | R: TTTTGGCAATATGGAAAGGC | Â |
#36 | GTAGTG | F: ATCCTATACGACAGCTCCGC | Â |
| Â | Â | R: TTTGGTGGCCATCTTCTTTC | Â |
#37 | ACA | F: AACTACAACAACAACGGCCA | Â |
| Â | Â | R: AAGCTTGTGAATTCGTTGCC | Â |
#38 | CAC | F: CACCACCCTTTCCACACTTC | Â |
| Â | Â | R: AATGAAGACCCTGCACCAAG | Â |
#39 | TTC | F: AGTCCTCCTCCTCCTCCTTG | Â |
| Â | Â | R: TGGGAGAACAAACAACACCA | Â |
#40 | AAT | F: TCAAAGGATAAGAATTATGGA | Â |
| Â | Â | R: CTGTTTACGCAGCACTTCCA | Â |
- Note: Primer pairs #1-#20 were used for 29 accessions genotyping; Primer pairs #1-#40 were used for diploid C. nankingense and C. lavandulifolium and their autotetraploid;
: Polymorphic between C. nankingense diploid and tetraploid plants; 
: Polymorphic between C. lavandulifolium diploid and tetraploid plants.
: Polymorphic between C. nankingense diploid and tetraploid plants; 
: Polymorphic between C. lavandulifolium diploid and tetraploid plants.