Table 1 Values of kinetic parameters for the cleavage of protein substrates by different catalysts
Catalyst | Protein | pH | Temperature (°C) | kcat(10−4 s −1) | Km(10−3 M−1) | kcat/Km(s−1M−1) |
|---|---|---|---|---|---|---|
Cu-MOF | BSA | 9.0 | 50 | 6.28 ± 0.39 | 0.28 ± 0.04 | 2.23 ± 0.35 |
Cu-MOF | BSA | 9.0 | 70 | 20.98 ± 0.65 | 0.27 ± 0.02 | 7.73 ± 0.68 |
Cu-MOF | casein | 9.0 | 50 | 5.17 ± 0.56 | 1.16 ± 0.24 | 0.44 ± 0.09 |
Cu-MOF | casein | 9.0 | 70 | 9.76 ± 0.17 | 0.42 ± 0.02 | 2.32 ± 0.12 |
Cu(II)A-PSa | BSA | 9.5 | 50 | 8.0 | 0.92 | 0.87 |
Cu(II)B-PSa | BSA | 9.5 | 50 | 8.7 | 1.2 | 0.73 |
Cu(II)oxacyclenb | BSA | 9.5 | 50 | 0.19 ± 0.003 | 0.51 ± 0.03 | 0.036 ± 0.003 |
Cu(II)cyclenb | BSA | 9.5 | 50 | N/A | N/A | 0.0005 |
Cu(II)Cc | BSA | 9.5 | 50 | 3.89 ± 0.56 | 0.11 ± 0.03 | 3.61 ± 0.83 |
Cu(II)Dc | BSA | 9.5 | 50 | 2.77 ± 0.277 | 0.52 ± 0.1 | 0.56 ± 0.11 |
Cu(II)Ec | BSA | 9.5 | 50 | 1.89 ± 0.17 | 0.17 ± 0.03 | 1.14 ± 0.19 |
Cu(II)Hc | BSA | 9.5 | 50 | 0.42 ± 0.06 | 0.14 ± 0.04 | 0.31 ± 0.083 |
Cu(II)Ic | BSA | 9.5 | 50 | 0.47 ± 0.06 | 0.53 ± 0.16 | 0.09 ± 0.024 |
