Figure 2
Examination of the tumor cell and Thy1 promoter activity co-localization.
(A–C): Transmitted light, pseudo-green and red fluorescent microscopic image of the tumor formed 35 days after 4T1-red tumor cell injection into the ear pinna of BALB/c(Thy1-YFP) transgenic mice. Photographs of the mice ears were made under light anaesthesia, using a Nikon Eclipse E600 fluorescent microscope. The YFP fluorescence in BALB/c(Thy1-YFP) mice shows co-localization with the red fluorescence of RFP-expressing tumor cells (4× magnification objective lens). (D–G): Transmitted light (F), green (D), red (E) fluorescent and combined (G) microscopic image of the sections of the Thy1-YFP mice ear 7 days after tdTomato expressing 4T1 tumor cell injection into the ear pinna. The red fluorescence of tumor cells is only partly co-localized with YFP fluorescence revealing the different origins of the cells comprising the tumor body. Photographs of the sections were made using a Zeiss Axiovision Z1 fluorescent microscope (20× magnification objective lens).
