Figure 2

Glucose-d₇ is utilized for lipid synthesis in pancreatic cancer PANC1 cells.

PANC1 cells were incubated with (a). 25 mM glucose-d₇ or (b). 25 mM glucose in glucose-free DMEM media supplemented with 10% FBS for 3 days. SRS imaging was taken at C–D vibration (~2120 cm⁻¹), off-resonance (~2600 cm⁻¹) or C–H vibration (~2850 cm⁻¹). (c). Raman spectra acquired from lipid droplets in control cells or glucose-d₇ treated cells. (d). Nontoxicity test of glucose-d₇ compared to regular glucose. Cell viability was determined by MTT assay. Data was represented as Mean + SD with n = 6 for each group.