Figure 2 | Scientific Reports

Figure 2

From: Genome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor

Figure 2

Production of EGFP fusion proteins in Fib-H-I1.

(a, b) Schematic representation of the transgenic vector. The detailed information of the vector construction was as described as Zhao et al.16 (c) The microinjection results and the fluorescent images of 3xp3-DsRed transgenic marker expression in the nerves system of embryo and compound eyes of adult. (d) Fluorescent images of dissected silk glands of N4-R3, N4/Fib-H-I1-R3 and Fib-H-I1-R3 during the wandering stage. (e) SDS-PAGE of cocoon proteins of N4 (1), N4/Fib-H-I1 (2), Fib-H-I1 (3), N4-R3 (4), N4/Fib-H-I1-R3 (5), Fib-H-I1-R3 (6) and (f) Western blotting analysis of cocoon proteins of N4-R3, N4/Fib-H-I1-R3 and Fib-H-I1-R3 with EGFP polyclonal antibody. Proteins were run at a 6% gel. The scale bars represent 1 cm.

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