Figure 7
From: In vitro molecular evolution yields an NEIBM with a potential novel IgG binding property
Comparison of IgG purification from mouse monoclonal ascites, human serum and rabbit serum with D-C-G3, SpA and SpG affinity chromatography.
(A) SDS/PAGE analysis of different IgG purified by D-C-G3, SpA and SpG affinity chromatography. M, protein molecular weight marker; 1–3, mIgG2a purified by DCG3, SpA, SpG affinity chromatography, respectively; 4–6, mIgG3; 7–9, mIgG2b; 10–12, mIgG1; 13–15 human IgG; 16–18, goat IgG purified by DCG3, SpA, SpG affinity chromatography, respectively. (B) Recovery of IgG from monoclonal antibodies, human serum and rabbit serum purified by different affinity chromatography. Black bars, purified IgG with DCG3 affinity chromatography; grey bars, purified IgG with SpA affinity chromatography; white bars, purified IgG with SpG affinity chromatography.
