Table 2 Surface plasmon resonance analysis of the interactions between D-C-G3, SpA, SpG or D-C and hIgG, rIgG, gIgG, mIgG1, mIgG2a, mIgG2b or mIgG3
From: In vitro molecular evolution yields an NEIBM with a potential novel IgG binding property
| Â | Â | Analyte | ||||||
|---|---|---|---|---|---|---|---|---|
Ligand | Constant | mIgG1 | mIgG2a | mIgG2b | mIgG3 | Human IgG | Rabbit IgG | Goat IgG |
DCG3 | ka(M−1s−1) | 1.318 × 105 | 1.445 × 106 | 2.168 × 105 | 3.364 × 105 | 1.318 × 106 | 4.132 × 105 | 3.147 × 105 |
|  | kd(s−1) | 1.923 × 10−4 | 1.444 × 10−5 | 1.389 × 10−4 | 1.511 × 10−4 | 8.790 × 10−7 | 2.377 × 10−5 | 6.520 × 10−5 |
|  | KA(M−1) | 6.85 × 108 | 1.00 × 1011 | 1.56 × 109 | 2.23 × 109 | 1.50 × 1012 | 1.74 × 1010 | 4.83 × 109 |
DC | ka(M−1s−1) | 3.474 × 104 | 1.554 × 106 | 6.789 × 104 | 6.783 × 105 | 1.161 × 106 | 6.118 × 105 | 1.238 × 105 |
|  | kd(s−1) | 4.283 × 10−4 | 5.696 × 10−4 | 3.890 × 10−4 | 5.378 × 10−4 | 2.762 × 10−5 | 1.069 × 10−4 | 5.662 × 10−4 |
|  | KA(M−1) | 8.11 × 107 | 2.73 × 109 | 1.75 × 108 | 1.26 × 109 | 4.20 × 1010 | 5.72 × 109 | 2.19 × 108 |
SpA | ka(M−1s−1) | 6.034 × 104 | 1.531 × 106 | 1.142 × 105 | 2.516 × 105 | 9.63 × 105 | 6.470 × 105 | 1.295 × 105 |
|  | kd(s−1) | 3.698 × 10−4 | 3.155 × 10−4 | 3.578 × 10−4 | 3.142 × 10−4 | 1.156 × 10−5 | 2.300 × 10−5 | 8.497 × 10−4 |
|  | KA(M−1) | 1.63 × 108 | 4.85 × 109 | 3.19 × 108 | 8.01 × 108 | 8.34 × 1010 | 2.81 × 1010 | 1.52 × 108 |
SpG | ka(M−1s−1) | 9.295 × 104 | 1.089 × 106 | 1.212 × 105 | 2.346 × 105 | 1.747 × 106 | 1.240 × 106 | 7.160 × 105 |
|  | kd(s−1) | 7.426 × 10−4 | 5.762 × 10−4 | 5.983 × 10−4 | 4.723 × 10−4 | 2.615 × 10−5 | 3.190 × 10−5 | 1.243 × 104 |
|  | KA(M−1) | 1.25 × 108 | 1.89 × 109 | 2.03 × 108 | 4.97 × 108 | 6.68 × 1010 | 3.88 × 1010 | 5.76 × 109 |
KADCG3/KADC | 8.45 | 36.64 | 8.94 | 1.76 | 35.70 | 3.04 | 22.26 | |
KADCG3/KASpA | 4.20 | 20.6 | 4.90 | 2.78 | 17.99 | 0.62 | 31.69 | |
