Figure 4
DMiroS182A,S324A,T325A or Loss of PINK1 Increases Mitochondrial Movement.
(A) Representative red/green overlay of two time-lapse images (Δ = 20 s) of accumulative JC1 staining in mitochondria at NMJs of muscle 6/7 hemisegment A3 of third instar larvae. Lower panels extract pixels that are present in only one image. (B) Quantification of the area of non-overlapping pixels between the two overlay images as shown in (A) divided by the total area of JC1 staining in each overlay image. n = 6–11 larvae. (C) Mitochondrial movement labeled by mito-GFP driven by CCAP-GAL4 in representative axons passing segment A3. The first frame of each live-imaging series is shown above a kymograph generated from the movie. The x axis of each is mitochondrial position and the y axis corresponds to time (moving from top to bottom). Vertical white lines represent stationary mitochondria and diagonal lines are moving mitochondria. (D) From kymographs as in (C), the percent of time each mitochondrion in motion was determined and averaged. n = 87–206 mitochondria from 8–12 axons and 5 animals. Scale bars: (A) 5 μm; (C) 10 μm. PINK1null: PINK5/Y31. PE704: precise excision control males for PINK1 null31. PINK1null_PINK1: PINK5/Y;+;da-GAL4_UAS-PINK1 (males of a rescue control for PINK1 null).
