Figure 8
The comparisons commercial dipstick and cotton-based diagnostic devices on the urinalysis assays by visual observation and quantitative.
Photographic evidence comparing commercial strip test and cotton-based diagnosis of a urine sample shows that the cotton-based diagnostic device changed color (yellow to green) as visible by the naked-eye in spiked BSA (3.75 μM) sample. The total quantitative procedure was almost the same as the protocol shown in Figure 3. After data readout (using a desktop scanner to scan the reacting test pad and turning the colorimetric output signal into a grayscale value to analyze the color intensity via the graphics processing software, ImageJ), we obtained the sensitivity to generate an Orginpro 8 histogram. Each value is the mean of three replicates (samples number N = 3) and the error bars represent the deviations of the measurements. The histogram illustrates the comparisons commercial dipstick and cotton-based diagnostic devices calibration plot for the mean intensity (I = spiked BSA 3.75 μM - normal) of the colorimetric results.
