Figure 2

WEF suppresses migration and invasion of B16F10 cells.

(A): Cells grown to 90% confluence were treated with mitomycin C for 30 min and then wounded by scraping. Cells were incubated in the presence of 25, 50 and 100 μg/ml WEF and migration was monitored using phase-contrast microscopy after 18 and 36 h. Based on the width of wound at 0 h, relative width at 18 and 36 h was calculated and expressed as the mean ± SD of four selected fields. (B): Cells pre-treated with 25, 50 and 100 μg/ml WEF for 12 h were subjected to migrate and invade through Transwell for 24 h and 36 h, respectively. Cells migrated and invaded to the lower surface of the Transwell membrane were stained and relative migration and invasion were quantified using ImageJ software. Data are representative of three independent experiments and expressed as the mean ± SD of five random fields of each well. #, p<0.01 vs. untreated control.