Figure 3

Oral administration of WEF inhibits pulmonary metastasis of B16F10 cells.
Five-week-old female C57BL/6J mice were intravenously injected via tail vein with B16F10 cells (3 × 105). After daily oral administration with saline (control) or WEF (50 mg/kg) for 17 days, mice were sacrificed and black colonies settled on the lung surface counted macroscopically after fixation. Images show metastatic colonies on the front (F) and back (B) side of the lungs. Metastatic colonies were counted and represented as the mean ± SD of each group (each group, n = 4). #, p<0.05 vs. control.