Figure 2 | Scientific Reports

Figure 2

From: A genetic variant of miR-148a binding site in the SCRN1 3′-UTR is associated with susceptibility and prognosis of gastric cancer

Figure 2

Characterization and functional analyses of the 3′-UTR of SCRN1.

(A) Schematic representation of reporter plasmids containing the SCRN1 3′-UTR, which was inserted downstream of Renilla luciferase gene in the psiCHECK™ -2 vector. (B) Complementarity between miR-148 and the SCRN1 3′-UTR site targeted. The SNP rs6976789 was located within the ‘seed region’ of the miR-148a binding site. (C) The effect of SNP rs6976789 on the interaction between the SCRN1 3′-UTR and miR-148a in MGC-803 and BGC-823 cells. The luciferase activity of each construct was normalized against the negative control miRNA (NC) transient transfected with constructed vectors with G allele.

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