Figure 3 | Scientific Reports

Figure 3

From: Combination of mild hypothermia with neuroprotectants has greater neuroprotective effects during oxygen-glucose deprivation and reoxygenation-mediated neuronal injury

Figure 3

Co-treatments reduced reactive oxygen species (ROS) generation after OGD/R.

Processing of primary cortical neurons were performed as above. The ROS were detected with DCFH-DA (Sigma, St. Louis, MO) staining by Spectra Max M5 Multi-Detection Microplate Readers (Molecular Devices, Sunnyvale, CA). The bars represent means ± s.d. (n = 4).*, P<0.05 compared with the other groups and **, P<0.05, compared with one or two groups. MH combined with HUK,MK-801 or Ngb significantly reduced intracellular ROS generation after OGD/R. (a) The combination of HUK and MH reduced the ROS generation of primary cortical neurons after OGD/R and was superior to HUK alone (t = 8.055, P<0.001) or MH alone (t = 7.849, P<0.001). (b) The combination of MK-801 and MH reduced the ROS generation of primary cortical neurons after OGD/R and was superior to agent (t = 11.581,P<0.001) or MH alone (t = 6.542, P<0.001). (c) The combination of Ngb and MH reduced the ROS generation of primary cortical neurons after OGD/R and was superior to agent (t = 13.569, P<0.001) or MH alone (t = 5.356, P<0.001).

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