Figure 4
Co-treatments reduced intracellular free calcium concentration ([Ca2+]i) after OGD/R.
Processing of primary cortical neurons were performed as above. The intracellular calcium concentration was determined with Fluo-3 (Biotium, Hayward, CA) staining and detected by FACS. The bars represent means ± s.d. (n = 4).*, P<0.05 compared with the other groups and **, P<0.05, compared with one or two groups. MH combined with HUK,MK-801 or Ngb significantly decreased [Ca2+]i after OGD/R. (a) The combination of HUK and MH reduced the intracellular calcium concentration in primary cortical neurons after OGD/R, to 1,817.500 ± 51.157, was superior to HUK alone (t = 14.901, P<0.001) or MH alone (t = 18.294, P<0.001). (b) The combination of MK-801 and MH reduced the intracellular calcium concentration in primary cortical neurons after OGD/R, was superior to MK-801 alone (t = 35.284, P<0.001) or MH alone (t = 30.352,P<0.001). (c) The combination of Ngb and MH reduced the intracellular calcium concentration in primary cortical neurons after OGD/R, was superior to Ngb alone (t = 9.374, P = 0.004) or MH alone (t = 7.101, P = 0.002). Abbrevation: [Ca2+]i :intracellular free calcium concentration.
