Figure 5
Co-treatments protected Mitochondrial Membrane Potential after OGD/R by reducing 530/590 ratio.
Processing of primary cortical neurons were performed as above. The ratio of the neurons with depolarized MMP to that with normal MMP was determined with JC-1 (Beyotime, Shanghai, China) staining and by FACS. Red staining (λex: 520 nm; λem: 590 nm) represented JC-1 aggregation with normal MMP, green staining (λex: 490 nm; λem: 530 nm) represented JC-1 monomer with depolarized MMP. The bars represent means ± s.d. (n = 4). *, P<0.05 compared with the other groups and **, P<0.05, compared with one or two groups. MH combined with HUK,MK-801 or Ngb significantly decreased 530/590 ratio in OGD/R neurons. (a) The combination of HUK with MH decreased green/red (530/590) ratio and was less than that treated with HUK (t = 37.534, P<0.001) or MH alone (t = 12.352, P<0.001). (b) The combination of MK-801 with MH decreased green/red (530/590) ratio and was less than that treated with MK-801 (t = 33.216, P<0.001) or MH alone (t = 21.345, P<0.001). (c) The combination of Ngb with MH decreased green/red (530/590) ratio and was less than that treated with Ngb (t = 14.795, P<0.001) or MH alone (t = 13.473, P<0.001). Abbrevation: ex: excitation, em: emission.
