Figure 2 | Scientific Reports

Figure 2

From: Development and application of loop-mediated isothermal amplification for detection of the F167Y mutation of carbendazim-resistant isolates in Fusarium graminearum

Figure 2

LAMP detection of the F167Y mutation in F. graminearum and digestion of positive LAMP products.

(A) LAMP for detection of the F167Y mutation using HNB as a visual indicator. The reaction becomes sky blue if the β2-tubulin gene has a point mutation at codon F167Y but remains violet if the β2-tubulin gene has no mutation or other mutation at codon F167Y. 1, ddH2O; 2, 2021; 3, R9. (B) Agarose gel electrophoresis of LAMP products. The positive reaction is manifested as a ladder-like pattern on the 3.0% agarose gel. M, 100-bp ladder; 1, ddH2O; 2, 2021; 3, R9. (C), LAMP products were digested with Pvu I and two fragments (119 bp, 88 bp) were observed by 3.0% agarose gel. M, 100-bp ladder, 1, LAMP products without digestion; 2, LAMP products digested by Pvu I.

Back to article page