Figure 3 | Scientific Reports

Figure 3

From: Development and application of loop-mediated isothermal amplification for detection of the F167Y mutation of carbendazim-resistant isolates in Fusarium graminearum

Figure 3

Optimization of LAMP reaction temperature (A, B) and reaction time (C, D).

Assessment was based on HNB visualization of color change in (A) and (C) and on gel electrophoresis in (B) and (D). M = 100-bp ladder. In (A) and (B), 1 = 60°C, 2 = 61°C, 3 = 62°C, 4 = 63°C, 5 = 64°C and 6 = 65°C. In (C) and (D), 1 = 15 min, 2 = 30 min, 3 = 45 min, 4 = 60 min and 5 = 90 min.

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