Figure 5 | Scientific Reports

Figure 5

From: Development and application of loop-mediated isothermal amplification for detection of the F167Y mutation of carbendazim-resistant isolates in Fusarium graminearum

Figure 5

Design of LAMP primers for detection of the F167Y mutation in F. graminearum.

(A) Nucleotide sequence alignment of the target region β2-tub in wild-type isolate 2021 and MBC-resistant isolate R9. The sequences used for LAMP primers are indicated by bold lines. One point mutation in red frame (TTT → TAT) leads to a moderate level of MBC resistance. (B) Schematic representation of the LAMP primers used in this study. Construction of the inner primers FIP and BIP are shown. F1c and B1c are complementary to F1 and B1, respectively.

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