Figure 3
Effect of cVA exposure, during mating or to synthetic cVA, on subsequent cVA attraction and female receptivity.
(A) Amount of cVA extracted from females after mating with fed (n = 8) and starved males (n = 6). Single females were dropped in hexane, immediately after mating and extracted during 5 min or 24 h. Amounts found on females mated with fed and starved males were analyzed with a Mann-Whitney test (** p < 0.01). (B) Virgin females were exposed to 0.3 or 0.6 μg of synthetic cVA during 1 h and then tested for cVA attraction. Asterisks above bars indicate significant attraction (Wilcoxon test, *** p < 0.001; data are mean ± SEM). (C) Receptivity of unmated females. Females were exposed to 0 (hexane), 0.3, 0.6 or 1.2 μg of cVA (n = 44, 40, 43 and 41, respectively) during 1 h, before they came in contact with a random virgin fed male during 1 h. Data were analyzed using a Mixed-effect model followed by a multiple comparison test (for details see SI Material and Methods , *** p < 0.001). Photos by S. Lebreton.
