Figure 5
From: Inhibition of breast cancer growth and metastasis by a biomimetic peptide
Inhibition of tumor-conditioned media induced spontaneous metastasis by SP2043.
Tumor-conditioned media (TCM) pre-treatment followed by tumor inoculation induce accelerated metastasis formation. SP2043 was administered (10 mg/kg, i.p.) during the TCM induction phase and the resulting effect on metastasis was observed for 5 weeks. (a) Number of mice with thoracic metastasis determined every week. (b) Number of organs with metastases ex vivo at week 5. (c) Mean photon flux from the LN and lungs. Luciferase expressed by MDA-MB-231 cells emit luminescent signal in the presence of D-luciferin (*P < 0.05, **P < 0.01). (d) Representative images of the harvested organs. (e) LN metastases. Anti-human vimentin antibodies detect metastatic human breast cancer cells in the LN. LN from animals treated with TCM showed macro-metastases (>2 mm); SP2043 treatment potently prevents LN metastasis. Scale bars represent 1,000 μm. (f) Colocalization of the metastases and lymphatic vessels in the LN. Scale bars in the whole images represent 1,000 μm. Enlarged images also showed this colocalization. The scale bar in the enlarged image represents 200 μm. Data (c) are reported as mean ± s.e.m.
