Figure 5
LysM-Cre; Tak1f/f mice showed a defect in osteoclastogenesis.
(a) LysM-Cre; Tak1f/f monocytes showed a reduction in differentiating into TRAP positive osteoclasts. Bone marrow suspension cells were cultured in the presence of RANKL and M-CSF for 7 days and were then stained for TRAP. (b) Quantitation data of TRAP positive multinucleated osteoclast (≥3). N = 3. **p < 0.01 when the value of mutant cells was compared to that of control cells. (c) LysM-Cre; Tak1f/f monocytes showed reduced expression of Integrin β3 and TRAP. Osteoclast cultures were collected three days after addition of RANKL and M-CSF and total RNA was isolated from these cultures and realtime PCR was carried out to determine the mRNA levels of Integrin β3 (left panel) and TRAP (right panel). N = 3. **p < 0.01 when the value of mutant cells was compared to that of control cells.
