Figure 6
Tak1 regulates osteoclast differentiation via multiple transcription factors and signaling pathways.
(a) The reduced mRNA levels of various transcription factors involved in osteoclast differentiation. Osteoclast cultures were collected three days after addition of RANKL and M-CSF and total RNA was isolated from these cultures and realtime PCR was carried out to determine the mRNA levels of these transcription factors. N = 3. *p < 0.05, **p < 0.01 when the value of mutant cells was compared to that of control cells. (b) Reduced activation of Tak1 downstream signaling pathways in Tak1 deficient monocyte/osteoclast cultures. Osteoclast cultures were collected three days after addition of RANKL and M-CSF and cells were harvested and Western blot was carried out to determine the levels of these activated signaling molecules. For the full-length blots see Supplementary Figure S3. (c) Analysis of the signaling events in the spleen of LysM-Cre; Tak1f/f mice. Spleens were homogenized and the samples were analyzed by western blot to determine the activation the signaling molecules. For the full-length blots see Supplementary Figure S4.
