Figure 7
Tak1 deficient monocytes/osteoclasts showed increased activity to promote osteoblastogenesis.
(a) LysM-Cre; Tak1f/f mice showed an increase in the number of ALP positive CFU. Bone marrow cells were isolated and red blood cells were lysed. The cells were washed with PBS and counted. 5 × 106 cells were plated into each well of the 6 well plates and were cultured in α-MEM with 10% serum. The plates were stained for ALP after plated for 7 days. Right panel: Quantitation data. N = 5. **p < 0.01 when the value of mutant cells was compared to that of control cells. (b) LysM-Cre; Tak1f/f monocytes showed a better activity to support MSC osteogenic differentiation in co-culture assays. Wild type MSC cells were plated and cultured for 24 hrs. Freshly bone marrow monocytes isolated from LysM-Cre; Tak1f/f and control mice were plated on top of the MSC cultures, without RANKL or M-CSF. After 5 days, the cells in suspension were washed off and the adherent MSC cells were stained for ALP. N = 3. **p < 0.01 when the value of mutant cells was compared to that of control cells.
