Figure 4

Separating chromatograms of MIP1 column and MIP2 column.

(A) was the chromatogram of the separation of CA from RDNI by MIP1 column. The chromatographic conditions were as follows: 0 ~ 50 min: CH₃OH-HOAc (500:1, v/v), after 50 min: CH₃OH-HOAc (9:1, v/v); the flow rate of mobile phase was 2.4 mL min⁻¹; the detective wavelength was 324 nm. (B) was the chromatogram of the separation of CACs from RDNI by MIP2 column. (C) and (D) were chromatograms of CA solution and CGA solution by MIP2. The mobile phase was CH₃OH-HOAc-HCOOH (100:1:3, v/v/v); flow rate of mobile phase which controlled by gravity was about 1.5 mL min⁻¹; the detective wavelength which subject to the conditions of the instrument was 340 nm.