Figure 3
From: Regulation of synaptic plasticity and cognition by SUMO in normal physiology and Alzheimer's disease
Inhibition of SUMO conjugation impairs hippocampal-dependent learning and memory.
(a) Schematic diagram shows the placement of cannulas reaching the dorsal hippocampi. (b) Contextual fear conditioning behavioral task. Cannulated mice were infused 1 hour prior to foot shock training. Vehicle n = 16, TAT-Ubc9 n = 8, TAT-Ubc9(DN) n = 16, TAT-GFP n = 12. ANOVA of % freezing behavior 24 hours after foot shock training p = 0.04; *p < 0.05 versus all other groups. (c) Contextual fear conditioning behavioral task. Cannulated mice were infused immediately after foot shock training. Vehicle n = 10, TAT-Ubc9 n = 12, TAT-Ubc9(DN) n = 8, TAT-GFP n = 6. ANOVA of % freezing behavior 24 hours after foot shock training p = 0.0002; **p < 0.01 versus all other groups. (d) Morris water maze behavioral task. Cannulated mice were infused after each training session. Vehicle n = 11, TAT-Ubc9 n = 11, TAT-Ubc9(DN) n = 12, TAT-GFP n = 11. ANOVA of time to reach the hidden platform p < 0.0001; *TAT-Ubc9(DN) group p < 0.05 versus all other groups starting from the third session. (e) Water maze probe trial. Mice infused with TAT-Ubc9(DN) spent significantly less time in the target quadrant (TQ). Adjacent right (AR), opposite quadrant (OQ), adjacent left (AL). ANOVA of time in target quadrant p < 0.0001; **TAT-Ubc9(DN) group p < 0.01 versus all other groups. Data presented as means ± SEM. See also Supplementary Figure S3.
